Northern B.C. salmon are the focus of a new study that may revolutionize how we count seasonal runs, and expand our understanding of populations in streams too remote to include in today含羞草研究社檚 surveys.
The study is a collaboration between Simon Fraser University and the Gitanyow Fisheries Authority (the technical arm of the Gitanyow Hereditary Chiefs near Terrace) with the goal of determining if salmonid environmental DNA (eDNA) found in river-water samples can establish the number of spawning fish as accurately as manual counts.
含羞草研究社淚 think we含羞草研究社檒l be successful. We含羞草研究社檙e looking at five different species and I think we含羞草研究社檝e got a really good chance at this,含羞草研究社� said lead researcher, SFU biology professor Vicki Marlatt.
Environmental DNA refers to traces of DNA animals shed into their surroundings 含羞草研究社� a pink salmon, for example, will release billions of cells per day in its feces and dislodged scales. For this study, researchers will collect one-litre samples of river water on a daily basis then count the DNA occurrences of the targeted species. With the help of a statistician, what含羞草研究社檚 found in that sample can be extrapolated to account for the entire stream.
含羞草研究社淭here含羞草研究社檚 a fair bit of math behind it,含羞草研究社� Marlatt said.
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The challenges are to figure out exactly how many cells each salmon species sheds in one day, to avoid counting an individual fish more than once, and then calibrating the process to account for ever-changing river conditions. But if the math is right, the count will be equal to the conventional manual method, where salmon are tallied one by one as they pass through fences and gates spanning the river.
The benefit of eDNA sampling means a low-cost, non-invasive method of counting that doesn含羞草研究社檛 require constant human presence. It can also be expanded for wider commercial use across fisheries and aquaculture sectors.
含羞草研究社淚f we含羞草研究社檙e successful, this would allow us to collect more data on the number of salmon in hundreds of rivers that are not being counted in Canada,含羞草研究社� Marlatt said. 含羞草研究社淎lso, if this proves to be more effective and efficient, in terms of human time and cost, then certainly this is something we could consider replacing some of the fish fences with.含羞草研究社�
But eDNA sampling would augment, not replace, the fence counts on the Kitwanga River.
Gitanyow Fisheries head biologist and senior technical advisor, Mark Cleveland, explained the fence off the mid-point of the Skeena River is the only one among few in the Northwest that counts all five species of salmon. It含羞草研究社檚 a state-of-the-art device with a high-definition camera to assist with manual counts that also enables staff to isolate about five per cent of the passing fish for important physical inspections for weight, sex and overall health.
含羞草研究社淭his is a unique facility 含羞草研究社� but it含羞草研究社檚 really labour intensive and expensive so you can含羞草研究社檛 have them everywhere,含羞草研究社� Cleveland said.
含羞草研究社淲ith this eDNA technology, 10 years down the road we might have it dialed in so we can go take water samples in different watersheds 含羞草研究社� if you take it at key times, you could do more surveys in more areas and it would result in better management.含羞草研究社�
Accurate counts are critical for Fisheries and Oceans Canada to determine allowable harvests each year in the commercial, recreational and First Nations fisheries. The department will sometimes rely on fence counts when river conditions disrupt efforts at the Tyee Test Fishery in the lower Skeena.
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含羞草研究社淚t含羞草研究社檚 important to reach that balance where we含羞草研究社檙e escaping enough fish to the various places so we don含羞草研究社檛 over-exploit them to the point they don含羞草研究社檛 come back. These programs give us the spot check we need,含羞草研究社� Cleveland said.
The eDNA study was scheduled for completion this year but high rains forced its postponement to 2021.
Marlatt is confident of the experiment含羞草研究社檚 success next year, citing one of very few related studies recently completed in Alaska.
含羞草研究社淚t really is a lot of work, and it was going so well,含羞草研究社� Marlatt said. 含羞草研究社淲e managed to get this up and running despite a pandemic and despite getting all the funds laid down and the team together 含羞草研究社� then the heavy rains came and the fence had to be opened otherwise the [river] would have taken it down. But we含羞草研究社檙e ready to go next year and I含羞草研究社檓 pretty optimistic, based on the study in Alaska. With some tight manual counts and tight water-flow data, I think we含羞草研究社檒l make some big strides.含羞草研究社�
The study is funded through Genome BC含羞草研究社檚 Sector Innovation Program.
